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Cellular pathologyCellular pathology is the branch of general pathologystudying the cellularbasis of disease. Various normal functions of cell growth, metabolism, and divisioncan fail or work in abnormal ways and lead to various diseases such as cancers. Various topics are studied by cellular pathologists:
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methods of study: - living tissue a) cell and tissue culture in an aseptic/sterile place has an incubation period of approximately 37 degrees celsius. using isotope labelling b)there is also bacteriology using bacteria cultured in agar plates. using tissue samples that are cultured for example Tuberculosis. - heat kills bacteria/cells it is used in the process of bacteriology and cytology (sputum) - freeze liquid gases such as carbon dioxide and nitrogen are used to freeze bacterias, cells and tissues after freezing the process of cryotomy is used in order to cut a slice of frozen tissue. - fix a) immersion in fluids (fixative) most common form of fixative are aldehydes for example formaldehyde and gluteraldehyde b) perfusion is used in research for optimum quality of results c) fixatives in a gaseous state liquids are heated to produce gas, this reduces solubilisation delocalisation and/or loss of cells or constituents.
Why Fix? - retain a life like quality(vivo like) - stop autolysis( self destruction by enzymes) - stop putrefacation(bacterial growth) - render the tissue suitable for subsuquent procedures.
there are many types of fixatives avaliable they are:- 1. coagulant's examples are Ethanol, Acetone, Piciric acid and mercuric chloride 2. non-coagulant's examples are Formaldehyde 3. Aldehyde's examples are formaldehyde and gluteraldehyde 4. Oxidising agents examples are osmium tetroxide, potassium permanganate and potassium dichronate. 5.Protein Denturing Agents examples are acetric acid, methanol and ethanol. 6. Other's piciric acid, mecuric acid. Categories: Medicine stubs| Cell biology| Pathology
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