Bradykinin

Bradykinin is a physiologically and pharmacologically active peptideof the kiningroup of proteins, consisting of nine amino acids.

Structure

The amino acidsequence of bradykinin is: arg- pro- pro- gly- phe- ser- pro- phe- arg. Its empirical formula is therefore C₅₀H₇₃N₁₅O₁₁.

Synthesis

The activity of the kinin-kallikrein systemmakes bradykinin by proteolytic cleavageof its kininogen precursor, high-molecular weight kininogen(HMWK), using the kininogenase enzyme.

Metabolism

In humans, bradykinin is broken down by three kininases: Angiotensin Converting Enzyme (ACE), aminopeptidase P (APP), and carboxypeptidase N (CPN), which cleave the 7-8, 1-2, and 8-9 positions, respectively ^{[{{fullurl:Template:FULLPAGENAME}}#endnote_1] [{{fullurl:Template:FULLPAGENAME}}#endnote_2]}.

Physiological role

Effects

Bradykinin is a potent endothelium-dependent vasodilator, causes contraction of non-vascular smooth muscle, increases vascular permeabilityand also is involved in the mechanism of pain. In some aspects, it has similar actions to that of histamine, and like histamine is released from venulesrather than arterioles.

Receptors

In mammals, two types of bradykinin receptorsare known. The B₁ receptor is only expressed as a result of tissue injury, and is presumed to play a role in chronic pain. The B₂ receptor is constitutively active and participates in bradykinin's vasodilatory role.

History

Bradykinin was discovered by three Brazilianphysiologists and pharmacologists working at the Instituto de Biologia de São Paulo, in São Paulo city, led by Dr. Maurício Rocha e Silva. Together with colleagues Wilson Teixeira Beraldoand Gastão Rosenfeldthey discovered in 1948 its powerful hypotensiveeffects in animal preparations. Bradykinin was detected in the blood plasmaof animals after the addition of venomof Bothrops jararaca (Brazilian lanceheadsnake), which was brought by Rosenfeld from the Butantan Institute. This discovery was part of a continuing study on circulatory shockand proteolyticenzymesrelated to the toxicologyof snake bites, started by Rocha e Silva as early as 1939. Bradykinin was to prove a new autopharmacologicalprinciple, i.e., a substance that is released in the body by a metabolic modification from precursors, which are pharmacologically active. According to B.J. Hagwood, Rocha e Silva's biographer, "The discovery of bradykinin has led to a new understanding of many physiological and pathological phenomena including circulatory shock induced by venoms and toxins."

Applications

The practical importance of the discovery of bradykinin became apparent when one of his collaborators at the Medical School of Ribeirão Preto, Dr. Sérgio Henrique Ferreira, discovered a bradykinin potentiating factor(BPF) in the bothropic venom which increases powerfully both the duration and magnitude of its effects on vasodilation and the consequent fall in blood pressure. On the basis of this finding, Squibbscientists developed the first of a new generation of highly-effective anti-hypertensive drugs, the so-called ACE inhibitors, such as captopril(trademarked Capoten), which have saved many lives since.

References

• ^  Dendorfer A, Wolfrum S, Wagemann M, Qadri F, Dominiak P. Pathways of bradykinin degradation in blood and plasma of normotensive and hypertensive rats. Am J Physiol Heart Circ Physiol 2001;280:H2182-8. Fulltext. PMID 11299220.
• ^  Kuoppala A, Lindstedt KA, Saarinen J, Kovanen PT, Kokkonen JO. Inactivation of bradykinin by angiotensin-converting enzyme and by carboxypeptidase N in human plasma. Am J Physiol Heart Circ Physiol 2000;278(4):H1069-74. Fulltext. PMID 10749699.de:Bradykinin

pt:Bradicinina

This article is licensed under the GNU Free Documentation License.
It uses material from the http://en.wikipedia.org/wiki/Bradykinin Wikipedia article Bradykinin.